LOCUS       B4GALNT3-pGEn2          8163 bp    DNA     circular     20-OCT-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6791..7332
                     /note="WPRE"
     misc_recomb     6753..6775
                     /label=attB2
                     /note="attB2 site"
     misc_feature    complement(7339..7553)
                     /note="BGH pA"
     misc_feature    complement(7641..7770)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3870..3890
                     /note="TEV site"
     misc_feature    3891..6752
                     /note="OPTIMIZED B4GALNT3 coding region"
     misc_feature    6558..6558
                     /note="Base insertion"
     vector          join(6761..8163,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..6760
                     /source="B4GALNT3-pDONR"
                     /type="Entry Clone"
     source          1..8163
                     /dnas_title="B4GALNT3-pGEn2 "
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTCTG AGAACCTGTA CTTCCAAGGC TCTGCCCAAG
     3901 TCGGCGGCAA CCCTCTGAAT AGAAGATACG GCTCTTGGAG AGAGCTGGCT AAGGCCCTGG
     3961 CCAGCAGAAA TATTCCCGCC GTGGATCCCC ATCTGCAGTT CTATCACCCT CAGCGGCTGA
     4021 GCCTGGAAGA TCACGATATT GATCAGGGCG TGTCCAGCAA CAGCAGCTAC CTGAAGTGGA
     4081 ACAAGCCCGT GCCTTGGCTG AGCGAGTTCA GAGGCAGAGC CAATCTGCAC GTGTTCGAGG
     4141 ATTGGTGCGG CAGCTCTATC CAGCAGCTGC GGAGAAATCT GCACTTCCCT CTGTACCCTC
     4201 ACATCCGGAC CACACTGAGA AAGCTGGCCG TGTCTCCCAA GTGGACCAAC TACGGCCTGC
     4261 GGATCTTTGG CTATCTGCAC CCCTTCACCG ACGGCAAGAT CCAGTTTGCT ATCGCCGCCG
     4321 ACGACAACGC CGAGTTTTGG CTGTCTCTGG ATGATCAGGT GTCCGGACTG CAACTGCTGG
     4381 CCTCTGTGGG AAAGACCGGC AAAGAATGGA CAGCCCCTGG CGAGTTCGGC AAGTTCAGAA
     4441 GCCAGATCAG CAAGCCTGTG TCTCTGAGCG CCAGCCACCG GTACTATTTC GAGGTGCTGC
     4501 ACAAGCAGAA CGAAGAGGGC ACCGATCATG TGGAAGTGGC CTGGCGAAGA AACGACCCTG
     4561 GCGCCAAGTT CACCATCATC GATAGCCTGA GCCTGTCTCT GTTCACCAAC GAGACATTCC
     4621 TCCAGATGGA CGAAGTGGGA CACATCCCTC AGACAGCCGC CAGCCATGTG GATAGCTCTA
     4681 ACGCCCTGCC TAGAGATGAG CAGCCTCCTG CTGATATGCT GCGGCCCGAT CCTAGAGACA
     4741 CCCTGTACAG AGTGCCTCTG ATCCCCAAGA GCCACCTGAG ACACGTGCTG CCTGATTGCC
     4801 CCTACAAGCC TAGCTACCTG GTGGATGGAC TGCCACTGCA GAGATACCAG GGCCTGAGAT
     4861 TCGTGCACCT GAGCTTCGTG TACCCCAACG ACTACACCAG ACTGAGCCAC ATGGAAACCC
     4921 ACAACAAGTG CTTCTACCAA GAGAACGCCT ACTACCAGGA CCGGTTCAGC TTCCAAGAGT
     4981 ACATCAAGAT CGACCAGCCT GAGAAGCAGG GCCTCGAGCA GCCTGGCTTT GAGGAAAATC
     5041 TGCTGGAAGA GTCCCAGTAC GGCGAGGTGG CAGAAGAGAC ACCAGCCAGC AACAACCAGA
     5101 ACGCCAGAAT GCTGGAAGGC AGACAGACCC CTGCCAGCAC ACTGGAACAG GACGCCACCG
     5161 ATTACCGGCT GAGATCTCTC AGAAAGCTGC TGGCCCAGCC AAGAGAAGGA CTGCTGGCTC
     5221 CCTTCAGCAA GAGAAACAGC ACAGCTAGCT TCCCCGGCAG AACCTCTCAC ATTCCTGTGC
     5281 AGCAGCCCGA GAAGCGGAAG CAGAAACCTT CTCCAGAGCC TAGCCAGGAC AGCCCTCACT
     5341 CTGATAAGTG GCCTCCAGGA CACCCCGTGA AGAACCTGCC TCAGATGAGA GGCCCTAGAC
     5401 CTAGACCTGC CGGCGATTCT CCTAGAAAGA CCCAGTGGCT GAACCAGGTG GAAAGCTATA
     5461 TCGCCGAGCA GCGGAGAGGC GACAGAATGA GGCCACAAGC ACCTGGAAGA GGATGGCACG
     5521 GCGAAGAAGA AGTGGTTGCC GCTGCTGGAC AAGAAGGCCA GGTTGAAGGC GAGGAAGAGG
     5581 GCGAAGAAGA AGAAGAGGAA GAGGACATGA GCGAGGTGTT CGAGTACGTG CCCGTGTTCG
     5641 ACCCCGTGGT CAATTGGGAT CAGACCTTCA GCGCCCGGAA CCTGGATTTT CAGGCACTGA
     5701 GGACCGACTG GATCGACCTG AGCTGCAATA CCAGCGGCAA TCTGCTGCTG CCAGAGCAAG
     5761 AGGCCCTGGA AGTGACCAGA GTGTTCCTGA AGAAGCTGAA TCAGCGGAGC CGGGGCAGAT
     5821 ACCAGCTGCA GAGAATCGTG AACGTGGAAA AGCGGCAGGA CCAGCTGAGA GGCGGAAGAT
     5881 ACCTGCTGGA ACTGGAACTG CTCGAACAGG GCCAGAGAGT TGTGCGGCTG TCCGAATATG
     5941 TGTCTGCCCG CGGATGGCAG GGAATTGATC CTGCTGGCGG AGAGGAAGTG GAAGCCAGAA
     6001 ATCTCCAGGG ACTCGTGTGG GACCCTCACA ATAGACGCAG ACAGGTGCTG AACACCCGGG
     6061 CTCAAGAACC CAAACTGTGT TGGCCTCAGG GCTTTTCTTG GAGCCACAGA GCCGTGGTGC
     6121 ACTTCGTGGT GCCTGTGAAG AATCAGGCCA GATGGGTGCA GCAGTTCATC AAGGACATGG
     6181 AAAACCTGTT CCAAGTGACC GGCGATCCCC ACTTTAACAT CGTGATCACC GACTACAGCA
     6241 GCGAGGATAT GGACGTGGAA ATGGCCCTGA AGCGGAGCAA GCTGAGAAGC TACCAGTACG
     6301 TGAAGCTGAG CGGCAACTTC GAGAGATCTG CAGGACTGCA GGCCGGCATC GATCTGGTCA
     6361 AGGATCCCCA CAGCATCATC TTTCTGTGCG ACCTGCACAT CCACTTTCCA GCCGGCGTGA
     6421 TCGACGCCAT CAGAAAGCAC TGTGTGGAAG GCAAGATGGC CTTCGCTCCC ATGGTCATGA
     6481 GACTGCACTG TGGCGCTACA CCACAGTGGC CTGAAGGCTA CTGGGAAGTG AATGGCTTTG
     6541 GCCTGCTGGG CATCTACaAG CCTGACATGG ACAGGACTGG GAGCCTGAAC ACCAAGGAGT
     6601 TCCGAGACCG CTGGGGCGGG GAAGACTGGG AGCTGCTGGA CAGGATACTC CAAGCGGGCC
     6661 TGGACGTGGA GCGTCTCTCC CTCAGGAATT TCTTCCATCA TTTCCATTCC AAGCGAGGCA
     6721 TGTGGAGCCG TCGCCAGATG AAGACGCTGT AACCAGCTTT CTTGTACAAA GTGGTTGATA
     6781 GCTTGGATCC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA
     6841 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
     6901 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
     6961 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC
     7021 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT
     7081 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG
     7141 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC
     7201 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC
     7261 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT
     7321 TCCGCGTCTT CGAGATCTGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC
     7381 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA
     7441 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG
     7501 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGACTCGAGT
     7561 TAAGGGCGAA TTGCCGATTA GGATCTTCCT AGAGCATGGC TACGTAGATA AGTAGCATGG
     7621 CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG
     7681 CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG
     7741 GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA GAGCTCATGG CGCGCCTAGG
     7801 CCTTGACGGC CTTCCGCCAA TTCGCCCTAT AGTGAGTCGT ATTACGTCGC GCTCACTGGC
     7861 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
     7921 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG AAACGCCCTT
     7981 CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGAGCGCCC TGTAGCGGCC ACTCAACCCT
     8041 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
     8101 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT
     8161 TAG
//

